Thermo Fisher Scientific CD120a (TNF Receptor I) Monoclonal Antibody (H398), eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

-

Neutralization (Neu)

-

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

H398

Immunogen

Rec. Human TNF-RI

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10596797

Product Specific Information

Description: The monoclonal antibody H398 recognizes the extracellular part of the Tumor Necrosis Factor Receptor type I (TNF-RI) of the membrane-bound as well as the soluble receptor.

The monoclonal antibody H398 can be used for immuno assay, flow cytometry, immuno precipitation and Western blotting. Furthermore, the monoclonal antibody H398 is useful for immunohistology on frozen sections. The monoclonal antibody H398 is also useful as antagonistic antibody in functional studies. Be aware that the antibody competes with TNF-alpha.

Applications Tested: Flow Cytometry, Functional Studies (Blocking), Immunohistochemistry (Frozen).

Purity: >95%.

Target Information

TNFR1 (Tumor necrosis factor receptor 1) belongs to the tumor necrosis factor superfamily, and is one of the major TNF-alpha receptors. TNFR1 plays an important role in mediating, in cytokine mediated signaling, positive regulation of the NF-Kb pathway, positive regulation of angiogenesis, and negative regulation of gene expression. The extracellular domain of TNFR1 is also released into the circulatory system as soluble TNFR1 (sTNFR1). In humans, the TNFR1 gene is located on chromosome 12. Anti-apoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with TNFR1, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of TNFR1 were found to be associated with the autosomal dominant periodic fever syndrome, and the impaired receptor clearance is thought to be a mechanism of the disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.