Thermo Fisher Scientific E4BP4 (NFIL3) Monoclonal Antibody (MABA223), PE, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 2 publications 2 publications

Product Specifications

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MABA223

Immunogen

21H2-hNFIL3 (aa 163-462) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NFIL3 (E4BP4),uniProtId:Q16649-1,ncbiNodeId:9606,antigenRange:163-462,antigenLength:462,antigenImageFileName:12-9812-41_NFIL3_E4BP4_Q16649-1_House_mouse.svg,antigenImageFileNamePDP:12-9812-41_NFIL3_E4BP4_Q16649-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

PE PE PE

View additional formats

• Alexa Fluor 647
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_11044503

Product Specific Information

Description: This MABA223 monoclonal antibody recognizes human E4BP4 (NFIL3), a basic leucine zipper transcription factor first identified for its binding to the promoters of the adenovirus E4 protein and human IL-3. E4BP4 is involved in the transcriptional control of the mammalian circadian clock, motor neuron growth and survival, regulation of von Willbrand factor expression, and osteoblast function. In addition, it is required for the development of NK and NKT cells as well as CD8a+ dendritic cells. Although relatively high levels of mRNA are reported in resting NK and NKT cells, studies at eBioscience have observed expression of E4BP4 protein in lymphocytes only after activation.

Applications Reported: This MABA223 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MABA223 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00). This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.