Thermo Fisher Scientific BDNF Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

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Immunohistochemistry (Frozen) (IHC (F))

1-10 µg/mL

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ELISA (ELISA)

1-10 µg/mL

-

Neutralization (Neu)

2-10 µg/mL

-

Product Specifications

Species Reactivity

Human, Rat

Published species

Rat

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant human BDNF (aa 129-247). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:BDNF,uniProtId:P23560-1,ncbiNodeId:9606,antigenRange:129-247,antigenLength:247,antigenImageFileName:PA1-18363_BDNF_P23560-1_Sheep.svg,antigenImageFileNamePDP:PA1-18363_BDNF_P23560-1_Sheep_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1070636

Product Specific Information

A cross reactivity of less than 1% against mouse NGF, recombinant human NT3 or NT4/5 has been shown by one site ELISA. Known to react with BDNF from rat and human.

Use at 1-10 µg/mL for inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50).

Reconstitute with 500 µL of distilled water. After reconstitution keep aliquots at -20º for a higher stability, and at 4ºC with an appropriate antibacterial agent.

Target Information

BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer`s and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.