Thermo Fisher Scientific alpha-1 Antitrypsin Monoclonal Antibody (TMF1#4B5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

-

Flow Cytometry (Flow)

3-5 µg/1x10^6 cells

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Radioimmune Assays (RIA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

TMF1#4B5

Immunogen

Purified human a1-Antitrypsin

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10984685

Product Specific Information

MA5-14661 targets Alpha1-Antitrypsin in ELISA, IP, and RIA applications and shows reactivity with Human samples.

The MA5-14661 immunogen is purified human a1-Antitrypsin.

MA5-14661 detects Alpha1-Antitrypsin which has a predicted molecular weight of approximately 46 kDa.

Product MA514661 is a smaller package size of MIA1101 (formerly sold as a Seradyn product).

Target Information

Alpha-1-AT is synthesized in the liver and it acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leucocytic proteases, plasmin, and thrombin, which may be released during inflammatory reactions in the lung. In the absence of alpha-1-AT, these enzymes are not inhibited and they may digest pulmonary parenchyma. Alpha-1-AT deficiency is associated with chronic obstructive lung disease (emphysema) and less frequently with hepatic cirrhosis in infants and respiratory distress of the newborn. Increase in alpha-1-AT occurs as an acute phase response to tissue necrosis and inflammation. Serum level of alpha-1-AT is elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.