Thermo Fisher Scientific FOXP3 Monoclonal Antibody (22510)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

10 µg/mL

-

Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

22510

Immunogen

Synthetic peptide derived from residues 400 to the C-terminus of Human FOXP3. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:FOXP3,uniProtId:Q9BZS1-1,ncbiNodeId:9606,antigenRange:400-431,antigenLength:431,antigenImageFileName:MA5-14664_FOXP3_Q9BZS1-1_House_mouse.svg,antigenImageFileNamePDP:MA5-14664_FOXP3_Q9BZS1-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• HRP
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

IgG fraction

Storage buffer

PBS, pH 7.5

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10982943

Product Specific Information

Heat mediated antigen retrieval recommended prior to tissue staining.

This antibody is predicted to react with mouse based on sequence homology.

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.