Thermo Fisher Scientific CD40 Monoclonal Antibody (5C3), Super Bright 645, eBioscience

Applications

• Flow Cytometry (Flow): Tested at 5 µL (0.125 µg)/test
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Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 685
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 690
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The 5C3 monoclonal antibody reacts with human CD40, a 45–50 kDa type I transmembrane glycoprotein and member of the TNFR family. CD40 is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. It regulates B cell development and maturation by inducing Ig isotype-switching and, together with IL-4, protects B cells from apoptosis and promotes proliferation. Interaction with CD154 (gp39) on T cells is crucial for T-B cell communication and immune regulation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on normal human peripheral blood cells at ≤5 µL (0.125 µg)/test
• Recommended Cell Range: 10⁵–10⁸ cells/test
• Optimization: Titrate for best performance

Fluorochrome Information:
Super Bright 645 is excited with a 405 nm violet laser and emits at 645 nm. Recommended bandpass filter: 660/20. Ensure instrument compatibility.

Staining Buffer Recommendation:
Use Super Bright Complete Staining Buffer (Product # SB-4401) when using multiple Super Bright-conjugated antibodies to minimize non-specific interactions.

Handling and Fixation:

• Protect from light (photo-sensitive dye)
• Fixation options:
  • IC Fixation Buffer (Product # 00-8222)
  • 1-step Fix/Lyse Solution (Product # 00-5333)
• Fixed samples stable up to 3 days at 4°C in dark

Excitation: 405 nm
Emission: 645 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD40 is a single-chain glycoprotein belonging to the TNF/NGF receptor superfamily, homologous to CD30. It is present on all B cells except plasma cells, and on some epithelial cells, carcinomas, and lymphoid dendritic cells. CD40 is essential for immune and inflammatory responses, including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. It is regulated by the AT-hook transcription factor AKNA and interacts with adaptor protein TNFR2. CD40-CD40L interaction is implicated in amyloid-beta-induced microglial activation, relevant to Alzheimer’s disease. Two alternatively spliced transcript variants encoding distinct isoforms have been reported.

Diseases associated with CD40 dysfunction:

• Type 3 Hyper-IgM Immunodeficiency
• CD40 Ligand Deficiency

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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