Thermo Fisher Scientific Mouse PD-L1 (B7-H1) His-tag Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species

Mouse

Expression System

HEK293 cells

Amino acid sequence

Mouse PD-L1, amino acids Met1-Thr238 (Accession # NP_068693.1) with a C-terminal His-tag

Tag

His-tag

Molecular weight

26.3 kDa

Class

Recombinant

Type

Protein

Purity

>98 % as determined by SDS-PAGE. ≥90 % as determined by SEC-HPLC.

Endotoxin concentration

<1.0 EU/µg

Activity

Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PD-L1 at 1 µg/mL (100 µL/well) can bind mouse PD1 with a linear range of 6.25-400 ng/mL.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.4, with 0.01% Tween 80, 5% mannitol, 5% trehalose

Contains

no preservative

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient

Product Specific Information

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Target Information

Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.