Thermo Fisher Scientific Human Siglec-2 (CD22) His-tag Recombinant Protein
Human Siglec-2 (CD22) His-tag 재조합 단백질로, HEK293 세포에서 발현된 고순도 연구용 시약입니다. ELISA, WB, IHC 등 다양한 면역학적 분석에 사용되며, 기능적 결합 활성이 검증되었습니다. 동결건조 형태로 제공되며, 안정적 저장 및 재구성이 용이합니다.
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | HEK293 cells |
| Amino Acid Sequence | Human CD22 isoform beta, amino acids Met1–Arg687 (Accession # P20273-1) with a C-terminal His-tag |
| Tag | His-tag |
| Molecular Weight | 76.6 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥95% (SDS-PAGE), ≥90% (SEC-HPLC) |
| Endotoxin Concentration | <1.0 EU/µg |
| Activity | Binds anti-human CD22 Mab in ELISA; linear range 0.2–50 ng/mL |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient |
Product Specific Information
Reconstitution:
Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
CD22 (BL-CAM) is a type 1 integral membrane glycoprotein (130–140 kDa) expressed in the cytoplasm and membrane of B-lymphocytes. It appears early in B-cell differentiation alongside CD19 antigen. Membrane expression is limited to mature B cells (CD22+) and plasma cells (CD22−), making it useful in phenotyping mature leukemias. CD22 is strongly expressed in hairy cell leukemia and binds preferentially to α2,6-linked sialic acid. This recognition site can be masked by cis interactions on the same cell surface. Ligand-induced tyrosine phosphorylation regulates B-cell receptor signaling, acting both positively via Src family kinases and negatively via phosphatase recruitment. CD22 is positive in diffuse large B-cell lymphoma and nodular lymphocyte predominance Hodgkin’s lymphoma, but negative in classical Hodgkin’s lymphoma.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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