Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), APC, eBioscience
Thermo Fisher의 CD11b Monoclonal Antibody (M1/70), APC는 mouse 및 human CD11b 검출용으로 최적화된 항체입니다. Flow Cytometry에 적합하며, 0.125 µg/test 농도로 사용됩니다. APC 형광 표지로 높은 감도와 안정성을 제공하며, 4°C 암소 보관이 권장됩니다.
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Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), APC, eBioscience™
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 3 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | - | 3 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | 308 publications |
| in situ PLA (PLA) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M1/70 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469342 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 660, 700
- APC-eFluor 780
- Biotin
- Brilliant UV and Brilliant Violet series (395–805 nm range)
- eFluor 450, 506, 660
- FITC
- Functional Grade
- NovaFluor Blue and Yellow series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright series (436–780 nm)
- Custom conjugation available
Product Specific Information
The M1/70 monoclonal antibody reacts with mouse CD11b, a 165–170 kDa integrin alphaM. CD11b associates with CD18 to form the αMβ2 integrin (Mac-1), binding to CD54 (ICAM-1), C3bi, and fibrinogen.
Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes, and B-1 cells. The M1/70 clone also cross-reacts with human CD11b, enabling detection on human monocytes, granulocytes, and NK cells.
CD11b participates in adhesive cell interactions via ligand binding.
Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes, ≤0.125 µg/test (100 µL volume, 10⁵–10⁸ cells/test). Optimize by titration for best performance.
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD11b (integrin alpha-M, ITGAM) is a 165 kDa adhesion molecule that forms a heterodimer with CD18 (integrin beta-2), known as Mac-1 or CR3.
It is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia.
CD11b/CD18 binds ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X, mediating cell-cell and cell-matrix interactions.
Expression varies across tissues and increases during monocyte maturation.
Associated with systemic lupus erythematosus and ITGAM-related susceptibility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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