Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), Brilliant Violet 711, eBioscience
인간 CD20 항원을 인식하는 2H7 클론 단일클론 항체로, Brilliant Violet 711 형광체가 결합되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, B세포 활성화 연구에 적합합니다. 4°C 암소 보관, 메탄올 고정에는 부적합합니다.
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Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), Brilliant Violet 711, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.5 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2H7 |
| Conjugate | Brilliant Violet™ 711 |
| Excitation/Emission Max | 406/711 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2929079 |
Additional Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 615 / 737 / 805
- Brilliant Violet 421 / 605 / 650
- eFluor 450 / 506
- FITC
- NovaFluor Blue 610-70S / 660-120S
- NovaFluor Red 710 / 725
- NovaFluor Yellow 590 / 660
- PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation
Product Specific Information
Description:
The 2H7 monoclonal antibody reacts with human CD20, a 33–36 kDa transmembrane protein expressed by developing and mature B cells but not plasma cells. CD20 is suggested to play a role in B-cell activation.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended use: 5 µL (0.5 µg) per test (100 µL final volume).
Fluorochrome Information:
Brilliant Violet™ 711 (BV711) emits at 713 nm and is designed for cytometers equipped with a 405 nm violet laser. Ensure instrument compatibility.
Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.
Light Sensitivity:
Protect vial and stained samples from light due to photo-induced oxidation sensitivity.
Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark. BV711 is not compatible with methanol-based fixation.
Excitation/Emission/Laser:
407 nm / 713 nm / Violet Laser
Trademark Notice:
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.
Target Information
CD20 is a non-glycosylated surface phosphoprotein (33–37 kDa) expressed on mature and malignant B cells, some T lymphocytes, and follicular dendritic cells. It regulates calcium conductance, cell cycle progression, and B-cell proliferation. CD20 is associated with lipid rafts and remains at the cell surface after antibody ligation, making it a useful target for therapeutic B-cell depletion.
Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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