Thermo Fisher Scientific Alkaline Phosphatase (Placental)/PLAP Monoclonal Antibody (ALPP, 4109)
인체 PLAP 단백질을 인식하는 Mouse monoclonal 항체로, 면역조직화학(IHC-P)에 최적화되어 있습니다. 단백질 A/G로 정제되었으며, 4°C에서 보관합니다. 포르말린 고정 조직에서 항원 복구가 필요하며, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC-P) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ALPP, 4109 |
| Immunogen | Recombinant full-length human ALPP protein (Placental Alkaline Phosphatase, UniProt ID: P05187-1, amino acids 1–535) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- IHC (PFA-fixed samples): Incubate antibody for 30 minutes at room temperature.
- Antigen retrieval: Heat tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes.
Target Information
Placental Alkaline Phosphatase (PLAP) is a membrane-bound isoenzyme expressed in the placenta during the third trimester of gestation. It is involved in inflammatory regulation and matrix mineralization. PLAP is useful for identifying testicular germ cell tumors. PLAP-positive somatic cell tumors typically express epithelial membrane antigen (EMA). Deficiency or mutation of this enzyme is associated with hypophosphatasia, a disorder characterized by hypercalcemia and skeletal defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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