Thermo Fisher Scientific DDB2 Monoclonal Antibody (OTI2E12), TrueMAB
DDB2 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot에 적합합니다. Human DDB2(aa 1-250) 재조합 단백질로부터 유래하였으며, 동결건조 형태로 제공됩니다. 친화 크로마토그래피로 정제되었고, 연구용으로만 사용 가능합니다.
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Applications
- Western Blot (WB): Tested dilution 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2E12 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–250 of human DDB2 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20 °C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to reach a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, an additional desalting step is recommended (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Damaged DNA binding protein (DDB) is a heterodimer composed of two subunits, p127 (DDB1) and p48 (DDB2). The DDB heterodimer plays a key role in repairing DNA damaged by ultraviolet light.
DDB, also known as UV-damaged DNA binding protein (UV-DDB), xeroderma pigmentosum group E binding factor (XPE-BF), and hepatitis B virus X-associated protein 1 (XAP-1), binds to damaged cyclobutane pyrimidine dimers (CPDs).
Mutations in the DDB2 gene cause xeroderma pigmentosum group E (XPE), an autosomal recessive disease characterized by hypersensitivity to sunlight, increased skin cancer risk, and neurological abnormalities.
The HBV X protein interacts with DDB1, which may mediate HBx transactivation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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