Thermo Fisher Scientific NFKBIE Monoclonal Antibody (OTI6H8), TrueMAB
Human NFKBIE 단백질을 인식하는 Mouse IgG1 단클론 항체로 Western Blot과 IHC(Paraffin)에 적합. Lyophilized 형태로 제공되며, PBS buffer(8% trehalose) 포함. 연구용으로 NF-κB 신호 억제 관련 단백질 검출에 활용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6H8 |
| Immunogen | Full length human recombinant protein of human NFKBIE produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
NFKB1 or NFKB2 binds to REL, RELA, or RELB to form the NFKB complex.
The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which retain NF-kappa-B in the cytoplasm.
Phosphorylation by kinases (IKBKA or IKBKB) targets I-kappa-B proteins for ubiquitination and degradation, allowing NF-kappa-B activation.
Activated NF-kappa-B translocates into the nucleus and binds DNA at kappa-B-binding motifs (e.g., 5'-GGGRNNYYCC-3').
For certain genes, activation requires interaction with other transcription factors such as STAT6, AP1 (JUN), and NFAT (NFATC1).
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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