Thermo Fisher Scientific HP1 alpha Recombinant Rabbit Monoclonal Antibody (SR44-03)
HP1 alpha 단백질을 표적으로 하는 재조합 토끼 모노클로날 항체로, Western blot, IHC, ICC/IF에 적합합니다. 높은 특이성과 일관된 로트 간 품질을 제공하며, 동물 유래 성분이 없는 포뮬레이션으로 연구용에 최적화되어 있습니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SR44-03 |
| Immunogen | Recombinant protein within Human HP1 alpha (aa 101–191) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809312 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, mediating gene silencing. Mammalian methyltransferases that methylate histone H3 on lysine-9 create a binding site for HP1 proteins, which play roles in gene silencing and chromatin structure.
HP1’s chromodomain recognizes methylated histone H3 peptides, acting as a specific interaction motif for the methyl epitope on lysine-9. In Suv39h double-null mouse fibroblasts, heterochromatin association of HP1 is lost but restored upon reintroduction of catalytically active SUV39H1 HMTase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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