Thermo Fisher Scientific HA Epitope Tag Polyclonal Antibody, DyLight 405
DyLight 405로 결합된 HA Epitope Tag 폴리클로날 항체로, WB, ICC/IF, ELISA 등 다양한 응용에 적합함. Rabbit IgG 기반으로 높은 특이성과 감도를 제공. HA 태그 단백질 검출 및 정제에 유용하며, 연구용으로만 사용 가능.
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Thermo Fisher Scientific HA Epitope Tag Polyclonal Antibody, DyLight 405
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to aa 114–122 (Y-P-Y-D-V-P-D-Y-A-G) of hemagglutinin influenza conjugated to KLH using maleimide |
| Conjugate | DyLight™ 405 |
| Excitation / Emission Max | 400 / 423 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term; for long term store at -20°C, avoiding freeze/thaw cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against the HA epitope tag and is useful in determining its presence in overexpressed proteins in various assays. It recognizes the HA epitope tag (Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-Gly) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Target Information
HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study protein topology and complexes, identify associated proteins, and characterize low abundance or poorly immunogenic proteins when protein-specific antibodies are unavailable.
HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin and is commonly expressed as a tag to aid identification and purification of tagged proteins for functional analysis.
HA tag antibodies can be used to facilitate protein detection or co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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