Thermo Fisher Scientific VSV-G Epitope Tag Polyclonal Antibody, DyLight 405
VSV-G epito프 태그를 검출하기 위한 DyLight 405 결합 폴리클로날 항체. Western blot, ICC/IF, ELISA 등 다양한 응용에 적합. 높은 특이성과 감도를 제공하며, 장기 보관 시 안정적. 연구용으로만 사용 가능.
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Thermo Fisher Scientific VSV-G Epitope Tag Polyclonal Antibody, DyLight 405
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to aa 501–511 (Y-T-D-I-E-M-N-R-L-G-K) of vesicular stomatitis virus glycoprotein (VSV-G), conjugated to KLH using maleimide |
| Conjugate | DyLight™ 405 |
| Excitation / Emission Max | 400 / 423 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if solution is not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
This affinity purified antibody is directed against the VSV-G epitope tag and is useful for detecting its presence in overexpressed proteins in various assays.
It recognizes the VSV-G epitope tag (Tyr-Thr-Asp-Ile-Glu-Met-Asn-Arg-Leu-Gly-Lys) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
Target Information
Vesicular stomatitis virus (VSV) is an enveloped RNA virus from the Rhabdoviridae family, released from host cells via budding.
The glycoprotein (VSV G) contains a domain in its extracellular membrane-proximal stem required for efficient budding.
Sequence: YTDIEMNRLGK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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