Thermo Fisher Scientific IL-4 Recombinant Rabbit Monoclonal Antibody (JA86-10)
Recombinant rabbit monoclonal antibody recognizing human IL-4. Validated for WB, IHC, ICC/IF. Expressed in HEK293 cells with Protein A purification. Supplied as 1 mg/mL liquid in TBS buffer with glycerol and BSA. Suitable for research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JA86-10 |
| Immunogen | Synthetic peptide within human IL-4 aa 85–125 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2911611 |
Product Specific Information
- Positive Control: MCF-7, HeLa, D3, human colon carcinoma tissue, human spleen tissue
- Subcellular Location: Secreted
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells and serves as a ligand for the interleukin 4 receptor. IL-4 exhibits a broad range of biological actions and also binds to IL13, contributing to overlapping functions of these cytokines. STAT6, a signal transducer and activator of transcription, mediates IL-4’s immune regulatory signaling. The IL-4 gene, along with IL-3, IL-5, IL-13, and CSF2, forms a cytokine gene cluster on chromosome 5q, with IL-4 located near IL13. These genes are co-regulated by long-range regulatory elements across approximately 120 kilobases. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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