Thermo Fisher Scientific IL-12/IL-23 p40 Monoclonal Antibody (C17.8), PE, eBioscience

Thermo Fisher Scientific IL-12/IL-23 p40 Monoclonal Antibody (C17.8), PE, eBioscience™

Applications and Tested Dilution

• Flow Cytometry (Flow): 5 µL (0.125 µg)/test
• Publications: View 18 publications

Product Specifications

Available Formats

• Alexa Fluor 488
• Biotin
• eFluor 450
• eFluor 660
• Functional Grade
• PE-Cyanine7
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The C17.8 antibody reacts with the p40 subunit of IL-12 and IL-23 and neutralizes their bioactivity. For specific IL-23 neutralization, clone G23-8 (anti-mouse IL-23 p19) is recommended.
IL-12 is a 70 kDa heterodimeric cytokine composed of p40 and p35 chains, secreted by activated monocytes, macrophages, and dendritic cells. IL-23 is a heterodimer composed of p19 and p40 proteins, expressed by activated dendritic cells.

Applications Reported: IP, Western blot, ELISA, neutralization, and intracellular staining for flow cytometry.
Applications Tested: Pre-titrated for intracellular flow cytometry of stimulated mouse cells. Use 5 µL (0.125 µg) per test in 100 µL volume (10⁵–10⁸ cells/test).
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-12 is produced by dendritic cells, neutrophils, macrophages, and lymphoblastoid cells upon antigenic stimulation. It is a heterodimer encoded by IL-12A (p35) and IL-12B (p40).
IL-23 shares the IL-12B subunit and includes IL-23A (p19). IL-12 enhances vascular endothelial adhesion molecule-1 expression and promotes leukocyte migration and tumor necrosis.
IL-23 stimulates memory T-cells and is essential for Th17 cell function, inducing neutrophil chemokines and pro-inflammatory cytokines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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