Thermo Fisher Scientific Phospho-SIK1/SIK2/SIK3 (Thr182, Thr175, Thr221) Polyclonal Antibody
Phospho-SIK1/SIK2/SIK3 (Thr182, Thr175, Thr221) 항체로 인산화된 SIK 단백질 검출에 특이적입니다. Western blot 및 IHC(Paraffin)에서 사용 가능하며, 사람, 마우스, 랫트 시료에 반응합니다. Rabbit polyclonal IgG 형식으로, 1 mg/mL 농도의 액상 제품입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human SIK1 (Accession P57059), corresponding to amino acid residues around phosphorylated Thr182. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817313 |
Product Specific Information
Specificity: Antibody detects endogenous levels of QIK/QSK only when phosphorylated at Thr182/Thr175/Thr221.
Target Information
Serine/threonine-protein kinase (SIK1) is involved in multiple cellular processes including cell cycle regulation, gluconeogenesis, lipogenesis, muscle differentiation, and tumor suppression. It phosphorylates targets such as HDAC4, HDAC5, PPME1, SREBF1, and CRTC1/TORC1, thereby modulating CREB activity and metabolic pathways.
SIK1 acts as a tumor suppressor via p53-dependent anoikis and regulates sodium signaling through phosphorylation of PPME1 and ATP1A1. It also influences muscle gene expression and cardiomyogenesis by controlling HDAC4/5 and CDKN1C/p57Kip2.
SIK2 modulates insulin signaling by phosphorylating IRS1 (Ser-794).
SIK3 regulates mTOR signaling through DEPTOR degradation and contributes to chondrocyte differentiation during skeletogenesis. It also negatively regulates cAMP signaling by acting on CRTC2/TORC2 and CRTC3/TORC3, and prevents HDAC4 nuclear translocation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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