Thermo Fisher Scientific Pax2 Polyclonal Antibody, Biotin
Pax2 단백질을 인식하는 Biotin 결합 토끼 폴리클로날 항체로, Western blot, IHC, ELISA, IP에 사용 가능. 인간, 마우스, 랫드, 영장류 반응성. 합성 펩타이드(aa 255–305)로부터 제작, 친화 크로마토그래피로 정제. -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide taken within amino acid region 255–305 on human PAX2 protein |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The Pax genes are a family of transcription factors active in specific tissues during early embryonic development. Pax family members possess a DNA-binding domain encoded by the paired box, enabling them to execute genetic programs. Several Pax genes are associated with developmental mutations, including:
- PAX3: Associated with Waardenburg syndrome
- PAX6: Associated with Aniridia
- PAX2: Associated with Wilms tumor
During embryogenesis, PAX2 is expressed in the developing kidney, particularly in condensing metanephric mesenchyme and early epithelial structures. Expression of PAX2 decreases as the tubular epithelium matures. However, PAX2 expression persists in the undifferentiated epithelium of Wilms tumors, correlating with proliferation of poorly differentiated epithelial cells. Expression of the Wilms tumor suppressor protein WT1 coincides with down-regulation of PAX2, suggesting WT1 can directly repress PAX2 transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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