Thermo Fisher Scientific HLA-DOA Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:200–1:2,000

ELISA

• Tested Concentration: 1 µg/mL

Product Specifications

Product Specific Information

Immunogen sequence:
TKADHMGSYG PAFYQSYGAS GQFTHEFDEE QLFSVDLKKS EAVWRLPEFG DFARFDPQGG LAGIAAIKAH LDILVERSNR SRAINVPPRV TVLPKSRVEL GQPNILICIV DNIFPPVINI TWLRNGQTVT EGVAQTSFYS QPDHLFRKFH YLPFVPSAED VYDCQVEHWG LDAPLLRHWE LQVPIPPPDA ME

Target Information

Reacts with HLA-DO, a heterodimer formed by DNα and DObβ subunits in B cells.
DNα and DObβ are products of the non-classical class II genes, HLA-DNA and HLA-DOB, respectively.
DO forms tight complexes with DM in the endoplasmic reticulum and is sorted to lysosomal vesicles during antigen processing and presentation.
It is selectively expressed on antigen-presenting cells such as B cells, dendritic cells, and thymic epithelial cells.
DO enhances peptide loading efficiency and stabilizes DM at low pH, preserving its chaperone activity.
DO-DM complexes are more efficient than DM alone in protecting empty DR molecules.
Reports describe DO as a co-chaperone of DM.

HLA and MHC antibodies are crucial in Immunopeptidomics, aiding identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem mass spectrometry.

⚠ WARNING
This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.