Invitrogen™

Alexa Fluor™ 647 Microscale Protein Labeling Kit

Microscale Protein Labeling Kits provide a convenient means for attaching a fluorescent label to a small amount of antibody or자세히 알아보기

Microscale Protein Labeling Kits provide a convenient means for attaching a fluorescent label to a small amount of antibody or protein (20–100 μg). The kits are available in four Alexa Fluor™ colors (or biotin) and supply everything needed for three labeling and separation reactions.

Important Features of Microscale Protein Labeling Kits:
• Labeled proteins typically ready to use typically in 2 hours (∼30 minutes hands-on time)
• Optimized for 20–100 μg of protein with molecular weights between 12 and 150 kDa
• Purified using convenient spin filters with yields between 60 and 90%
• Stabilizing proteins must be removed from the sample before labeling

Stable Reaction Chemistry and Superior Alexa Fluor™ Dyes
In the Microscale Protein Labeling Kits, the reactive dye contains a succinimidyl (NHS) ester moiety that reacts with primary amines of proteins to form stable dye-protein conjugates. Compared to traditional dyes, Alexa Fluor™ dyes are brighter, more photostable, and more pH resistant between pH 4 and 10. And generally when using Alexa Fluor™ dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see [Alexa Fluor™ Dyes Spanning the Visible and Infrared Spectrum—Section 1.3](http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Fluorophores-and-Their-Amine-Reactive-Derivatives/Alexa-Fluor-Dyes-Spanning-the-Visible-and-Infrared-Spectrum.html Alexa Fluor Dyes Spanning the Visible and Infrared Spectrum—Section 1.3).

Learn More About Protein and Antibody Labeling
We offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental setup. See Antibody Labeling from A to Z or use our Labeling Chemistry Selection Tool for other choices. To learn more about our various kits read [Kits for Labeling Proteins and Nucleic Acids—Section 1.2](http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Fluorophores-and-Their-Amine-Reactive-Derivatives/Kits-for-Labeling-Proteins-and-Nucleic-Acids.html Kits for Labeling Proteins and Nucleic Acids—Section 1.2) in the Molecular Probes™ Handbook.

We`ll Make a Custom Antibody Conjugate for You
If you cant find what youre looking for in our stocked list, we`ll prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 13485:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

사양

제품 유형Labeling Kit

수량1 Kit

검출 방법Fluorescence

여기/방출650/665 nm

라벨링 방법Conjugation-based

라벨링 스케일20–100 μg

배송 조건Room Temperature

제품라인Alexa Fluor™

반응성 부분Succinimidyl (NHS) Ester

라벨 또는 염료Alexa Fluor 647

색상Far-Red

Labeling TargetProteins

라벨 유형Alexa Fluor

Unit SizeEach