Thermo Fisher Scientific H2AX Polyclonal Antibody
Rabbit polyclonal antibody targeting the C-terminus of human histone H2AX. Suitable for WB and IHC applications. Detects phosphorylated H2AX (γ-H2AX) related to DNA damage response. Supplied as a liquid at 1 mg/mL, stored at 4°C, shipped on wet ice.
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- A300083Ax (2개 옵션)
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | C-terminus of human histone H2AX |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Shelf Life: Recommended shelf life is 1 year from date of receipt.
Target Information
Histone H2A.X (H2AX) is a member of the histone H2A family, one of the four core histones forming the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) trigger phosphorylation of serine 139 at the C-terminus of H2AX, generating γ-H2AX. This phosphorylation can be detected by Western blotting or immunofluorescence to assess DSB frequency.
Phosphorylation of H2AX is mediated by phosphatidylinositol 3-kinases, including ATM, DNA-PK, and ATR. H2A.X consists of 143 amino acids and is synthesized in both G1 and S phases. Its mRNA exists in two forms: a short replication-type (575 bases) and a long basal-type (1600 bases). The gene maps to human chromosome 11q23.2–q23.3.
Histone H2A.X contributes to nucleosome structure and DNA repair by regulating MDC1 (mediator of DNA damage checkpoint protein 1) recruitment to DNA damage sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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