Thermo Fisher Scientific hnRNP L Monoclonal Antibody (4D11)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.25-0.5 µg/mL

-

Immunocytochemistry (ICC/IF)

5 µg/mL

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Non-human primate, Bovine

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4D11

Immunogen

Human hnRNP proteins from HeLa cells, purified by affinity chromatography on ssDNA agarose. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:hnRNP L,uniProtId:P14866-1,ncbiNodeId:9606,antigenRange:1-589,antigenLength:589,antigenImageFileName:MA1-26745_hnRNP_L_P14866-1_House_mouse.svg,antigenImageFileNamePDP:MA1-26745_hnRNP_L_P14866-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2248549

Product Specific Information

Recommended positive controls: MDBK.

A band of ~65 kDa can be detected.

Target Information

Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.