Thermo Fisher Scientific hnRNP A1 Monoclonal Antibody (9H10)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

5 µg/mL

-

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

RNA Immunoprecipitation (RIP)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Dog, Hamster, Human, Mouse, Non-human primate, Rat

Published species

Hamster, Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

9H10

Immunogen

Full length, native human hnRNPA1 protein partially purified from HeLa cells. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:hnRNP A1,uniProtId:P09651-1,ncbiNodeId:9606,antigenRange:1-372,antigenLength:372,antigenImageFileName:MA1-26736_hnRNP_A1_P09651-1_House_mouse.svg,antigenImageFileNamePDP:MA1-26736_hnRNP_A1_P09651-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_794496

Product Specific Information

Recommended positive controls: 293T.

A band of approximately 34 kDa is detected by Western Blot.

Target Information

HnRNP A1 belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre mRNAs in the nucleus and appear to influence pre mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. hnRNP A1 has two repeats of quasi RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re imported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.