Thermo Fisher Scientific Rat anti-Mouse IgG1 Secondary Antibody, PE, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 0.25 µg/test

Product Specifications

Available Conjugate Formats

• APC
• Biotin
• eFluor 450
• eFluor 570
• eFluor 615
• eFluor 660
• FITC
• HRP
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Custom conjugation available upon request

Product Specific Information

The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and is suitable as a secondary reagent in flow cytometry and microscopy.
It does not recognize other mouse isotypes or any rat immunoglobulins.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested by flow cytometric analysis of cells stained with a mouse IgG1 primary antibody
• Recommended Usage: ≤ 0.25 µg per test (100 µL final volume, 10⁵–10⁸ cells/test)
• Excitation: 488–561 nm
• Emission: 578 nm
• Compatible Lasers: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Anti-Mouse secondary antibodies are affinity-purified with high specificity for mouse immunoglobulins.
They are useful for detection, sorting, or purification of mouse antibodies and provide enhanced sensitivity through signal amplification.
Typically generated by immunizing the host animal with pooled mouse immunoglobulins, these antibodies can be further purified and modified (e.g., immunoaffinity chromatography, label conjugation) to produce highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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