Thermo Fisher Scientific VASP Polyclonal Antibody
VASP 단백질을 인식하는 Rabbit Polyclonal Antibody로 Western blot, IHC, ICC/IF 등에 사용 가능. 고순도(>95%) 항원 친화 크로마토그래피로 정제됨. 인간, 생쥐, 랫트 반응성. 연구용으로 세포 부착 및 이동 관련 단백질 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 200–250 of Human VASP |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2803369 |
Product Specific Information
Purity is >95% by SDS-PAGE.
Target Information
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. These proteins contain an EHV1 N-terminal domain that binds proteins with E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions.
The mid-region includes a proline-rich domain that binds SH3 and WW domain-containing proteins, while the C-terminal EVH2 domain mediates tetramerization and binds both G and F actin.
VASP is associated with filamentous actin formation and plays a role in cell adhesion and motility, as well as intracellular signaling pathways regulating integrin–extracellular matrix interactions. It is regulated by cyclic nucleotide-dependent kinases PKA and PKG.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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