Thermo Fisher Scientific Zap-70 Polyclonal Antibody
ZAP-70 단백질을 인식하는 Rabbit Polyclonal 항체로, 인간, 마우스, 랫트 시료에 반응합니다. Western blot, IHC, ICC/IF에 적합하며 고순도(>95%)로 정제되었습니다. 세포 신호전달 연구용으로 적합하며, PBS/glycerol buffer에 보존됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 300–350 of Human ZAP70 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2803371 |
Product Specific Information
Purity is greater than 95% by SDS-PAGE.
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family. It is expressed exclusively in T-cells and natural killer cells and plays a crucial role in T-cell receptor activation. Upon TCR engagement, ZAP70 is phosphorylated on tyrosines 292, 315, and 319 in the interdomain B region. Phosphorylation at tyrosines 315 (Vav binding site) and 319 (Lck binding site) enhances ZAP70 function in lymphocyte signaling, while phosphorylation at tyrosine 292 attenuates activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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