Thermo Fisher Scientific Phospho-VAMP4 (Ser30) Polyclonal Antibody
VAMP4 단백질의 Ser30 인산화 형태를 특이적으로 인식하는 폴리클로날 항체. Western blot, IHC, ICC/IF에 사용 가능. 사람, 생쥐, 랫트 반응성. PBS 기반 용액 형태로 제공되며 -20°C에서 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human VAMP4 (Accession O75379), corresponding to amino acid residues around phosphorylated Ser30 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817232 |
Product Specific Information
This antibody detects endogenous levels of VAMP4 only when phosphorylated at Ser30.
Target Information
The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium-binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane-associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer’s disease, VAMP levels of all isoforms appear to be significantly lowered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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