Thermo Fisher Scientific acnB Recombinant Rabbit Monoclonal Antibody (102)

Applications

ELISA

• Tested Dilution: 1:5,000–1:10,000

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide (final concentration 0.05%–0.1%) to prevent contamination.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.

Advantages of recombinant rabbit monoclonal antibodies:

• Superior specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulation
• Broader immunoreactivity due to the rabbit immune repertoire

This antibody specifically recognizes E. coli acnB.

Target Information

acnB is involved in the catabolism of short-chain fatty acids (SCFA) through the tricarboxylic acid (TCA) cycle and the 2-methylcitrate cycle I (propionate degradation route). It catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate and the hydration of 2-methyl-cis-aconitate to yield (2R,3S)-2-methylisocitrate.

The apo form of AcnB functions as an RNA-binding regulatory protein. During oxidative stress, inactive apo-AcnB (without iron-sulfur clusters) binds the acnB mRNA 3′ UTRs, stabilizing acnB mRNA and increasing AcnB synthesis, thus mediating a post-transcriptional positive autoregulatory switch. AcnB also decreases the stability of the sodA transcript.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.