Thermo Fisher Scientific VISTA Recombinant Rabbit Monoclonal Antibody (PSH0-64)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

PSH0-64

Immunogen

Recombinant protein within human vista aa 1-194 / 311. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:VISTA,uniProtId:Q9H7M9-1,ncbiNodeId:9606,antigenRange:1-194,antigenLength:311,antigenImageFileName:MA5-49363_VISTA_Q9H7M9-1_Rabbit.svg,antigenImageFileNamePDP:MA5-49363_VISTA_Q9H7M9-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3075020

Product Specific Information

Sequence Similarities: 95% Mouse/Rat.

Tissue Specificity: Expressed in spleen. Detected on a number of myeloid cells including CD11b monocytes, CD66b-positive neutrophils, at low levels on CD4-positive and CD8-positive T-cells, and in a subset of NK cells. Not detected on B cells (at protein level). Expressed at high levels in placenta, spleen, plasma blood leukocytes, and lung. Expressed at moderate levels in lymph node, bone marrow, fat, uterus, and trachea. Has low expression levels in other tissues.

Positive Control: Human brain tissue, human placenta tissue, human tonsil tissue, A375 cell lysate, HeLa cell lysate, human lung tissue lysate.

Subcellular Location: Cell membrane.

Predicted band size: 34 kDa.

Target Information

VISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-1H, is a product of the VSIR gene. Although VISTA bears sequence homology to PD-L1 it has a different expression pattern. In both mouse and humans, VISTA is expressed predominantly on hematopoietic cells with the highest densities on myeloid cells, and lower levels on T cells. It is usually absent on B cells. VISTA expressed by antigen presenting cells has been shown to directly suppress proliferation and cytokine production of CD4 and CD8 T cells, making it a target for cancer immunotherapy. The reported ligands of VISTA include CD28H, VSIG-3 and VSIG-8.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.