Thermo Fisher Scientific Alkaline Phosphatase Polyclonal Antibody, HRP

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000

Immunoprecipitation (IP)

• Tested Dilution: 1:500–1:2,000

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot contents and freeze at -20°C or below.
• Avoid repeated freeze/thaw cycles.
• Centrifuge if solution is not clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

Alkaline Phosphatase antibody is an IgG fraction purified from monospecific antiserum through delipidation, salt fractionation, ion-exchange chromatography, and dialysis.
Assay by immunoelectrophoresis shows a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, and purified Alkaline Phosphatase [Human Intestine].
Cross-reactivity with alkaline phosphatase from other sources may occur but has not been specifically determined.

Target Information

Alkaline phosphatase (ALP) is a homodimeric enzyme (~86 kDa) containing two zinc atoms per monomer essential for catalytic activity.
It is optimally active at alkaline pH and is found in both prokaryotes and eukaryotes.
ALP plays roles in liver metabolism and skeletal development.
In bacteria, it acts as a source of inorganic phosphate, while in yeast it aids in membrane protein sorting studies.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.