Thermo Fisher Scientific HSF1 Monoclonal Antibody (744509)

Applications

• Immunohistochemistry (Frozen) (IHC (F)): 8–25 µg/mL

Product Specifications

Product Specific Information

Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.

Target Information

All organisms respond to elevated temperatures and various environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator heat shock factor (HSF), which binds to heat shock response elements (HSEs).
HSEs consist of three repeats of a 5-nucleotide {nGAAn} module arranged in alternating orientations and are present upstream of all heat shock genes. Although HSEs are highly conserved, HSF proteins differ among species and show limited immunological cross-reactivity.

In human cells, HSF1 and HSF2 have been identified, sharing 38% sequence identity and binding to the same HSEs. HSF1 responds to classical stress signals (heat, heavy metals, oxidative reagents), while HSF2 is activated during hemin-mediated differentiation of erythroleukemia cells.
HSF1 exists constitutively in the cytoplasm and nucleus of unstressed cells as a monomer lacking DNA-binding activity. Upon stress, HSF1 transitions to a nuclear-localized trimeric state capable of DNA binding. Phosphorylation of HSF1 is required for maximal transcription of heat shock genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.