Thermo Fisher Scientific CD5 Monoclonal Antibody (53-7.3), NovaFluor Yellow 610, eBioscience

Thermo Fisher Scientific CD5 Monoclonal Antibody (53-7.3), NovaFluor Yellow 610

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.2 µg/test

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Custom conjugation available

Product Specific Information

The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67 kDa protein expressed on thymocytes, mature T cells, and a subset of B cells (B-1 cells). CD5 acts as a counter-receptor for CD72 and plays a role in T-B cell interactions.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes at ≤0.25 µg/test (100 µL final volume). Optimal titration recommended.

Note: NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be used for optimal staining with NovaFluor conjugates.

• Add 5 µL per sample before combining with cells.
• For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates are based on Phiton™ technology, which uses nucleic acid dye structures for engineered fluorescent signatures minimizing spillover and spread.

Excitation: 552 nm
Emission: 612 nm
Laser: 561 nm (Yellow)

Internal testing indicates non-specific B-cell staining in SJL mice; not observed in BALB/c or C57BL/6 strains.

Target Information

CD5 is a 67 kDa transmembrane glycoprotein found on mature T lymphocytes, thymocytes, and some B cells. It regulates T-cell proliferation and is expressed in many T-cell leukemias and lymphomas.
Associated diseases include thymus cancer and Richter’s Syndrome.

For Research Use Only. Not for use in diagnostic procedures.

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