
Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), Super Bright 600, eBioscience
마우스 Ly-6G(Gr-1)에 특이적으로 결합하는 1A8-Ly6g 단클론 항체로, Super Bright 600 형광염료로 표지되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 중성구 탐지 및 제거 연구에 활용 가능합니다. 405 nm 레이저로 여기되어 600 nm에서 방출합니다.
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Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), Super Bright 600, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 22 publications |
Product Specifications
| Specification | Description |
|---|---|
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 600, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A8-Ly6g |
| Conjugate | Super Bright™ 600 |
| Excitation / Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2762784 |
Available Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 563, 661, 737
- Brilliant Violet 421, 480, 650, 786
- eFluor 450
- FITC
- Functional Grade
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436, Super Bright 702
- Request custom conjugation
Product Specific Information
The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G (Gr-1), a 25-kDa GPI protein expressed exclusively by neutrophils. It plays a role in neutrophil infiltration, recruitment, and migration. Ly-6G associates with B2 integrins (CD11a, CD11b) and modulates their expression and function.
Unlike RB6-8C5, this clone binds only Ly-6G on neutrophils and can be used for neutrophil depletion.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse bone marrow cells at ≤0.5 µg/test (100 µL volume). Cell number: 10⁵–10⁸ cells/test. Optimal titration recommended.
Fluorochrome Information:
Super Bright 600 is a tandem dye excited at 405 nm and emits at 600 nm. Recommended filter: 610/20 bandpass. Use Super Bright Complete Staining Buffer (SB-4401) when combining multiple Super Bright dyes to minimize non-specific interactions.
Light Sensitivity: Protect from light.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333). Clone-specific performance should be verified empirically.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Ly6G (Lymphocyte antigen 6 complex locus G6D) is part of the myeloid differentiation antigen Gr-1, along with Ly6C.
- Molecular weight: 21–25 kDa
- Expressed by myeloid-derived cells in bone marrow
- Marker for peripheral neutrophils, monocytes, and granulocytes
- Correlates with granulocyte differentiation and maturation
- Involved in anti-tumor responses and modulation of local immune activity
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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