Thermo Fisher Scientific GARP Monoclonal Antibody (G14D9), Super Bright 600, eBioscience
인간 GARP 단백질을 인식하는 Rat IgG2a 단클론 항체로, Super Bright 600 형광체로 결합되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 활성화된 Treg 세포 표면의 GARP 발현 분석에 적합합니다. 빛에 민감하므로 어둡게 보관하십시오.
- 카탈로그번호
- 63-9882-42
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific GARP Monoclonal Antibody (G14D9), Super Bright 600, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.5 µg)/Test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 600, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | G14D9 |
| Immunogen | A mixture of mGARP-Fc and hGARP 21H-F1 & -F2 |
| Conjugate | Super Bright™ 600 |
| Excitation/Emission Max | 414/601 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2735047 |
Additional Formats
Product Specific Information
Description:
The G14D9 monoclonal antibody reacts with human Glycoprotein A Repetitions Predominant (GARP, also known as LRRC32 or Garpin). GARP is an ~80 kDa glycoprotein expressed on the cell surface. Expression has been reported in placenta, lung, kidney, heart, ovary, liver, skeletal muscle, and pancreas, as well as on megakaryocytes, platelets, and activated regulatory T (Treg) cells. GARP expression on activated Treg cells is essential for their suppressive function, possibly as a receptor for LAP/TGF-β.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. Use at 5 µL (0.5 µg) per test in a final volume of 100 µL. Recommended cell number: 10⁵–10⁸ cells/test.
Fluorochrome Information:
Super Bright 600 is a tandem dye excited by the violet laser (405 nm) and emits at 600 nm. Recommended filter: 610/20 bandpass. Ensure instrument compatibility.
Staining Recommendations:
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Light Sensitivity:
Sensitive to photo-induced oxidation. Protect from light.
Fixation:
Samples may be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal brightness loss.
Excitation/Emission: 405 nm / 600 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
GARP (Leucine Rich Repeat Containing 32, LRRC32) is a type I membrane protein with 22 leucine-rich repeats and a short intracytoplasmic region. Identified through Treg and Th cell gene expression profiling, it is expressed in CD4⁺, CD25⁺, FoxP3⁺ regulatory T cells and serves as a marker for Treg cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.