Thermo Fisher Scientific NEMO/IKK-gamma Polyclonal Antibody
Thermo Fisher Scientific의 NEMO/IKK-gamma Polyclonal Antibody는 인간 NEMO 단백질을 인식하는 토끼 IgG 항체로, WB, ELISA, IP에 사용 가능. 고순도 정제 및 안정한 액상 형태로 제공되며, NF-kappaB 관련 연구에 적합.
- 카탈로그번호
- 200-401-GM7x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific NEMO/IKK-gamma Polyclonal Antibody
Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:50,000–1:400,000 |
| Immunoprecipitation (IP) | 5 µg |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-NEMO was affinity purified from whole rabbit serum prepared by repeated immunizations with a recombinant protein of human NEMO. Target family: IKK gamma (UniProt ID: Q9Y6K9-1), antigen range: 1–419, antigen length: 419. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.
Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-NEMO is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography followed by extensive dialysis.
This antibody detects human NEMO. Cross-reactivity with NEMO from other sources is unknown.
Target Information
IKK gamma is the regulatory subunit of the inhibitor of the kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways.
Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other immunodeficiencies.
Multiple transcript variants encoding different isoforms have been found for this gene.
A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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