Thermo Fisher Scientific PARP1 (N-term ZF1) Polyclonal Antibody
인간 PARP1 단백질에 특이적인 폴리클로날 항체로, Western blot 및 IHC에 적합합니다. Rabbit IgG 형태로, Protein A로 정제된 액상 제품입니다. -20°C에서 보관하며 반복적인 동결/해동을 피해야 합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PARP1 (N-term ZF1) purified antibody prepared from whole rabbit serum produced by repeated immunizations with N-terminus region of human PARP1 zinc finger domain recombinant protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This antibody is specific for human PARP1 protein.
No cross-reactivity detected towards other PARP members when using siRNAs against 18 PARP family members.
Cross-reactivity with PARP1 from other sources has not been determined.
Target Information
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyze the transfer of ADP-ribose to various nuclear protein acceptors.
Proteolysis of PARP to its stable 85 kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein.
Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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