Invitrogen™

Anza™ 10-Pack Starter Kit

The Invitrogen™ Anza™ 10-Pack Starter Kit includes the 10 common restriction enzymes, 2 DNA modifying enzymes, and buffers listed below:•자세히 알아보기

The Invitrogen™ Anza™ 10-Pack Starter Kit includes the 10 common restriction enzymes, 2 DNA modifying enzymes, and buffers listed below:

• Anza™ 11 EcoRI
• Anza™ 5 BamHI
• Anza™ 1 NotI
• Anza™ 16 HindIII
• Anza™ 12 XbaI
• Anza™ 8 XhoI
• Anza™ 3 BcuI (isoschizomer to SpeI)
• Anza™ 10 DpnI
• Anza™ 19 BglII
• Anza™ 26 Eco32I (isoschizomer to EcoRV)
• Anza™ T4 DNA Ligase Master Mix
• Anza™ Alkaline Phosphatase
• Anza™ Buffer (10X)
• Anza™ Red Buffer (10X)

For a kit with just 5 enzymes, see the Anza™ 5-Pack Starter Kit.

The Anza™ Restriction Enzyme Cloning System employs a single buffer and protocol for all Anza restriction enzymes. The single Anza buffer allows multiple restriction enzymes to be used simultaneously in one reaction. This eliminates the need for sequential digests and the DNA clean-up necessary when changing buffers. Additionally, the Anza buffer and restriction enzymes have been designed for flexibility in digestion times. Digestions of up to 16 hours can be carried out without star activity.

• Convenient—all Anza enzymes are 100% functional in the single Anza buffer
• Fast—complete digestion in 15 minutes
• Flexible—overnight digestion without star activity
• Simple—a single digestion protocol for all DNA types
• Complete system—incorporates Anza DNA Modifying Enzymes for a complete cloning experience

Anza Red Buffer, which contains a density gradient and tracking dyes, is supplied for added convenience. Digestion reactions are as efficient in the Anza Red Buffer as in the Anza Buffer. Use of the Anza Red Buffer helps reduce pipetting steps when preparing multiple digestion reactions and a subsequent step includes agarose gel electrophoresis. For applications that require product analysis by fluorescence excitation, the colorless Anza Buffer without the tracking dyes is recommended.

The Anza 10-pk Starter Kit is a subset of the Anza Restriction Enzyme Cloning System, unifying the traditional cloning processes. For more information on the system, visit www.thermofisher.com/anza.

사양

배송 조건Approved for shipment on Wet or Dry Ice

메틸화 민감도Methylation Sensitivities Varies by Enzyme

효소HindIII, NotI, Alkaline Phosphatase, XhoI, BcuI, XbaI, DpnI, T4 DNA Ligase, Eco32I, BamHI, BglII, EcoRI

함께 사용가능한 버퍼Anza™ 10X Red Buffer, Anza™ 10X Buffer

Type IIS RENo

수량1 kit

제품 유형Starter Kit

연구 카테고리Traditional Cloning

제품라인Anza™

Unit SizeEach