Invitrogen™

Anza™ T4 DNA Ligase Master Mix

The Invitrogen Anza™ T4 DNA Ligase Master Mix facilitates the joining of abutted 5-phosphate and 3-hydroxyl termini in duplex DNA자세히 알아보기

The Invitrogen Anza™ T4 DNA Ligase Master Mix facilitates the joining of abutted 5-phosphate and 3-hydroxyl termini in duplex DNA through the formation of a phosphodiester bond. It can be used to join DNA fragments with both sticky ends and blunt ends, and to repair nicks in double-stranded DNA with 3-hydroxyl and 5-phosphate ends. Anza T4 DNA Ligase is formulated as a 4X concentrated master mix. Ligation can be performed with DNA in water, TE, elution buffer, or 1X Anza™ buffers.

Benefits:
• Ligation complete in 15 minutes at room temperature
• A single ligase master mix for both sticky-end and blunt-end ligation reactions
• Ready-to-use master mix format reduces pipetting steps
• 4X concentration enables more dilute DNA to be used in ligation reaction without concentrating

Anza T4 DNA Ligase Master Mix is part of the Anza™ Restriction Enzyme Cloning System, unifying the traditional cloning processes. Other Anza DNA Modifying Enzymes include the Anza T4 PNK Kit, Anza DNA Blunt End Kit, and Anza DNA End Repair Kit.

사양

농도4X

배송 조건Approved for shipment on Wet or Dry Ice

효소T4 DNA Ligase

함께 사용가능한 버퍼Elution Buffer, 1X Anza Buffer

수량50 Reactions

제품 유형T4 DNA Ligase Master Mix

제품라인Anza™

Unit SizeEach