Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1), Alexa Fluor 700, eBioscience

Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1), Alexa Fluor 700, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• Brilliant Violet 786
• eFluor 450
• Functional Grade
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Yellow 700
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
The MIH1 monoclonal antibody reacts with human B7-H1 (PD-L1), a member of the B7 family with a molecular weight of approximately 40 kDa. PD-L1 is expressed on most leukocytes and serves as a ligand for PD-1. Interaction of PD-1 with PD-L1 or PD-L2 inhibits T and B cell responses. MIH1 is reported to be a blocking antibody.

Applications Reported:
MIH1 antibody has been reported for use in flow cytometric analysis.

Applications Tested:
Pre-diluted and tested by flow cytometry on stimulated normal human peripheral blood cells. Recommended usage: 5 µL (1.0 µg) per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.

Fluorochrome Information:
Alexa Fluor 700 emits at 723 nm and is excited by the red laser (633–647 nm). Recommended optical setup: 685 LP mirror and 710/20 bandpass filter.

• Excitation: 633–647 nm
• Emission: 723 nm
• Laser: Red Laser

Target Information

Programmed death receptor ligand 1 (PD-L1, B7-H1) is a B7 family member that binds PD-1, negatively regulating T-cell receptor (TCR) signaling. PD-L1 ligation reduces TCR-mediated proliferation and cytokine production. PD-L1 is abundantly expressed in murine and human cancers and upregulated by IFN-γ stimulation, suggesting its role in tumor immune evasion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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