Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1), Alexa Fluor 700, eBioscience
인간 PD-L1(B7-H1)을 인식하는 MIH1 클론 단일클론 항체로, Alexa Fluor 700으로 표지되어 Flow Cytometry에 최적화됨. 633–647 nm 레이저로 여기, 723 nm에서 방출. 면역억제 경로 연구 및 종양 면역 회피 기전 분석에 활용 가능.
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Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1), Alexa Fluor 700, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 2 publications |
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunocytochemistry (ICC/IF) | - | View 4 publications |
| Flow Cytometry (Flow) | 5 µL (1.0 µg)/test | View 25 publications |
| Neutralization (Neu) | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 700 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MIH1 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2802351 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- APC-eFluor 780
- Biotin
- Brilliant Violet 786
- eFluor 450
- Functional Grade
- NovaFluor Red 685
- NovaFluor Red 700
- NovaFluor Yellow 700
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
Description:
The MIH1 monoclonal antibody reacts with human B7-H1 (PD-L1), a member of the B7 family with a molecular weight of approximately 40 kDa. PD-L1 is expressed on most leukocytes and serves as a ligand for PD-1. Interaction of PD-1 with PD-L1 or PD-L2 inhibits T and B cell responses. MIH1 is reported to be a blocking antibody.
Applications Reported:
MIH1 antibody has been reported for use in flow cytometric analysis.
Applications Tested:
Pre-diluted and tested by flow cytometry on stimulated normal human peripheral blood cells. Recommended usage: 5 µL (1.0 µg) per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.
Fluorochrome Information:
Alexa Fluor 700 emits at 723 nm and is excited by the red laser (633–647 nm). Recommended optical setup: 685 LP mirror and 710/20 bandpass filter.
- Excitation: 633–647 nm
- Emission: 723 nm
- Laser: Red Laser
Target Information
Programmed death receptor ligand 1 (PD-L1, B7-H1) is a B7 family member that binds PD-1, negatively regulating T-cell receptor (TCR) signaling. PD-L1 ligation reduces TCR-mediated proliferation and cytokine production. PD-L1 is abundantly expressed in murine and human cancers and upregulated by IFN-γ stimulation, suggesting its role in tumor immune evasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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