Thermo Fisher Scientific Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5), Alexa Fluor 700, eBioscience
마우스 Ly-6G/Ly-6C(Gr-1) 항원을 인식하는 RB6-8C5 클론의 Alexa Fluor 700 결합 단클론 항체입니다. 유세포분석에 최적화되어 있으며, 0.125 µg/test 권장. 696/719 nm 형광 특성, 4°C 보관, 연구용 전용 제품입니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 22 |
| Immunocytochemistry (ICC/IF) | - | 6 |
| Flow Cytometry (Flow) | 0.125 µg/test | 92 |
| Immunoprecipitation (IP) | - | 1 |
| Functional Assay | - | 3 |
| In Situ Hybridization (ISH) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Dog, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RB6-8C5 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_494008 |
Additional Available Formats
Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 561, Alexa Fluor 660, APC, APC-eFluor 780, Biotin, Brilliant UV series, Brilliant Violet series, eFluor series, FITC, Functional Grade, NovaFluor, PE, PE-Cyanine series, PerCP series, Super Bright series, and custom conjugation available.
Product Specific Information
The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21–25 kDa GPI-linked protein also known as the myeloid differentiation antigen Gr-1. Gr-1 is expressed by myeloid lineage cells in a developmentally regulated manner in the bone marrow.
Monocytes express Gr-1 transiently during development, while granulocytes and peripheral neutrophils show strong Gr-1 expression, making it a reliable marker for these populations.
eBioscience testing indicates that clone RB6-8C5 also stains cells expressing high levels of Ly6C. For Ly-6G specific detection, clone 1A8-Ly6G (Product #9668) is recommended.
Applications Reported: Flow cytometric analysis.
Applications Tested: Suitable for flow cytometry of mouse bone marrow and splenocyte suspensions at ≤0.125 µg/test (100 µL final volume). Optimal titration is recommended.
Fluorochrome Information:
Alexa Fluor® 700 emits at 723 nm and is excited with a red laser (633 nm). Recommended filter setup: 685 LP mirror and 710/20 filter.
Excitation: 633–647 nm; Emission: 723 nm; Laser: Red Laser.
Post-manufacturing filtered through 0.2 µm.
Target Information
Granulocytes are white blood cells characterized by cytoplasmic granules and lobed nuclei (polymorphonuclear leukocytes). Neutrophils are the most abundant type. These cells are released from bone marrow under regulation by complement proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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