Thermo Fisher Scientific ATG12 Polyclonal Antibody
ATG12 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot 및 IHC에 사용 가능. 합성 펩타이드(aa 20-70, Drosophila ATG12)로 면역화됨. 동결건조 형태로 제공되며, 재구성 후 4°C 또는 -20°C에서 보관. 오토파지 관련 단백질 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:300–1:2,000 | - |
| Immunohistochemistry (IHC) | 1:300–1:2,000 | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from aa region 20–70 of Drosophila ATG12 conjugated to blue carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not determined |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1089672 |
Product Specific Information
Reconstitute with 150 µL of distilled water.
Target Information
ATG12, a member of the autophagy protein family, forms a conjugate with ATG5, exhibiting ubiquitin-protein ligase (E3)-like activity for protein lipidation during autophagy.
This conjugate also interacts with innate immune response proteins such as RIG-I and VISA (IPS-1), inhibiting type I interferon production and facilitating viral replication.
ATG12 interacts with ATG10 in human embryonic kidney cells in the presence of ATG7.
At least two isoforms of ATG12 are known.
Autophagy, the process of bulk degradation of cellular proteins via the autophagosomic-lysosomal pathway, is crucial for normal growth control and may be defective in tumor cells.
It maintains cellular nutrients under starvation and regulates cytosolic component turnover.
This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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