Thermo Fisher Scientific Phospho-c-Raf (Ser339) Polyclonal Antibody
Phospho-c-Raf (Ser339) 폴리클로날 항체로, 인간·마우스·랫에서 반응하며 WB, IHC, ICC, ELISA 등 다양한 응용에 사용 가능. Protein A 정제, 1 mg/mL 농도, -20°C 보관. 세포 신호전달 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human c-Raf around the phosphorylation site of Ser339 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) functioning downstream of the Ras family of membrane-associated GTPases. Once activated, Raf-1 phosphorylates and activates MEK1 and MEK2, which in turn activate ERK1 and ERK2. Activated ERKs regulate gene expression involved in cell cycle, apoptosis, differentiation, and migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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