Thermo Fisher Scientific BrdU Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 3 publications 3 publications

Flow Cytometry (Flow)

1:50-1:200

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Product Specifications

Species Reactivity

Chemical

Published species

Chemical

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

BrdU conjugated to KLH

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2549729

Product Specific Information

Recommended positive controls: HeLa cells were treated with 100uM BrdU for 4hrs, U343 cells were treated with 10 µM BrdU for 18hrs.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.