Thermo Fisher Scientific LC3B Polyclonal Antibody
LC3B 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody입니다. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합하며, 인간 및 설치류 등 여러 종에 반응합니다. 오토파지 연구 및 신경발생 관련 연구에 활용 가능합니다.
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Thermo Fisher Scientific LC3B Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 5 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 1 publication |
| Flow Cytometry (Flow) | 1:50–1:200 | – |
| Immunoprecipitation (IP) | 1:100–1:500 | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Pig, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a region within amino acids 1–125 of Human LC3B |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.34 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2549727 |
Product Specific Information
- Recommended positive controls: NT2D1, PC-3, U87-MG, SK-N-SH, mouse brain, rat brain, HepG2 (untreated or Thapsigargin-treated), Huh7 (infected/uninfected), HeLa (Chloroquine-treated).
- Predicted reactivity: Zebrafish, Japanese Medaka, Pig, Rhesus Monkey, Chimpanzee, Bovine (all 100%).
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
LC3B (Autophagy Marker Light Chain 3B, MAP1A/MAP1B LC3 B) is encoded by the MAP1LC3B gene in humans.
LC3B is associated with microtubule assembly and plays a key role in neurogenesis and autophagy — a process responsible for cytoplasmic component degradation.
Macroautophagy involves the formation of double-membrane autophagosomes that fuse with lysosomes for degradation.
LC3B mediates interactions between microtubules and cytoskeletal components and is implicated in cancer, aging, metabolic, neurodegenerative, and cardiovascular/pulmonary diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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