Thermo Fisher Scientific EPCAM Monoclonal Antibody (OTI2A3), TrueMAB
EpCAM 단백질을 표적으로 하는 Mouse IgG1 단클론 항체로, Western blot, IHC, ICC 등 다양한 응용에 적합합니다. 인간 시료 반응성이 있으며, 무보존제 PBS 버퍼에 동결건조 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2A3 |
| Immunogen | Full-length human recombinant EPCAM protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent cell adhesion molecule and influences cell cycle, proliferation, and metabolism through induction of c-myc and cyclins A/E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated interactions, affecting epithelial differentiation and growth. Overexpression is linked to enhanced epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for epithelial lineage tumors.
Ep-CAM is found on the baso-lateral surface of most simple epithelia and many carcinoma types, and helps distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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