Thermo Fisher Scientific EPCAM Monoclonal Antibody (OTI2D5), TrueMAB

Applications

• Western Blot (WB)
  Tested Dilution: 1:4,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It mediates Ca²⁺-independent homotypic cell adhesion, influencing cell cycle regulation, proliferation, and metabolism through activation of genes such as c-myc, cyclin A, and cyclin E.
Ep-CAM expression negatively regulates cadherin-mediated adhesion, affecting epithelial differentiation and growth. Overexpression is associated with increased epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for tumors of epithelial origin. It is localized to the basolateral surface of most simple epithelia and many carcinoma types, and can distinguish adenocarcinomas from pleural mesotheliomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.