Thermo Fisher Scientific Human ATF6 Synthetic Peptide

Applications

Control (Ctrl)

• Assay-dependent

Blocking Assay (BLOCK)

• Assay-dependent

Product Specifications

Product Specific Information

This peptide corresponds to 17 amino acids near the amino terminus of human ATF6.
PEP-0349 can be used as a blocking peptide with polyclonal antibody PA5-20216.

Target Information

ATF6 is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. It binds DNA on the 5'-CCAC[GA]-3' half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires NF-Y binding. ATF6 may also participate in transcriptional activation via the serum response factor.

ATF6 forms homodimers and heterodimers with ATF6 beta, and interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC). It also interacts with transcription factors GTF2I, YY1, and SRF. Under ER stress, the cleaved N-terminal cytoplasmic domain translocates into the nucleus. The basic domain functions as a nuclear localization signal, while the basic leucine-zipper domain mediates NF-Y binding and ERSE recognition.

During the unfolded protein response, an approximately 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis, sequentially cleaved by site-1 and site-2 proteases. ATF6 is N-glycosylated, phosphorylated in vitro by MAPK14/P38MAPK, and belongs to the bZIP family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.