Thermo Fisher Scientific Phospho-PKM2 (Tyr83) Polyclonal Antibody
PKM2 단백질의 Tyr83 인산화 형태를 특이적으로 검출하는 Rabbit Polyclonal Antibody. Western blot과 IHC(Paraffin)에 적합하며, Human, Mouse, Rat 시료에서 반응. 고순도 정제 및 안정적 PBS/glycerol 저장 포뮬러로 연구용에 최적화.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human PKM (Accession P14618), corresponding to amino acid residues around phosphorylated Tyr83 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816925 |
Product Specific Information
Antibody detects endogenous levels of PKM2 only when phosphorylated at Y83.
Target Information
PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. PKM2 has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms of PKM2 have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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